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. 2011 Mar 31;8:150. doi: 10.1186/1743-422X-8-150

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Copyright ©2011 Gu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of HN protein expressed in the recombinant fowlpox virus (rFPV) by indirect immunofluoresence assay (IFA). Chick embryo fibroblast (CEF) cells were infected with rFPV-LaSHN (A), rFPV-JS6HN (B), or wild-type parental FPV (C) at a multiplicity of infection (MOI) of 0.01. Sixty hours post-infection, IFA was performed using polyclonal anti-NDV chicken serum and FITC-conjugated rabbit anti-chicken IgG as primary and secondary antibody, respectively. The fluorescence was observed under an inverted fluorescence microscope.