Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 May 24;6(5):e19791. doi: 10.1371/journal.pone.0019791

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Hoefling et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Comparison between measured FRET efficiency histograms (black) and histograms computed from the simulations (blue: ensemble, all-trans and cis01 ... cis20). Red dots denote the respective mean values. The simple model sketched on top and defined in the text describes the general trend (green line) that isomers with a cis-bond close to the termini show lower efficiencies, whereas those with cis-bonds close to the polymer center tend to yield higher efficiencies. The dashed green lines estimate the spread of the average efficiencies of the cis simulations mirroring the spread found for the all-trans simulations (). For illustration purposes, the photons of the individual cis were not discarded when generated below as described in the Methods Section. The high efficiencies observed for cis-6 to cis-12 result from dyes in contact and are quenched in the experiment.

Inline graphic — Comparison between measured FRET efficiency histograms (black) and histograms computed from the simulations (blue: ensemble, all-trans and cis01 ... cis20). Red dots denote the respective mean values. The simple model sketched on top and defined in the text describes the general trend (green line) that isomers with a cis-bond close to the termini show lower efficiencies, whereas those with cis-bonds close to the polymer center tend to yield higher efficiencies. The dashed green lines estimate the spread of the average efficiencies of the cis simulations mirroring the spread found for the all-trans simulations (). For illustration purposes, the photons of the individual cis were not discarded when generated below as described in the Methods Section. The high efficiencies observed for cis-6 to cis-12 result from dyes in contact and are quenched in the experiment.