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. 2011 May 24;6(5):e20143. doi: 10.1371/journal.pone.0020143

Figure 9. Western Blot analysis with LC3B-antibody in ASPC-1 and MiaPaCa-2 cells.

Figure 9

The experiment was repeated three times with nearly identical results. The LC3-I fractions can be clearly distinguished above the LC3-II signals. Bafilomycin A1 elevated slightly the LC3-II signal intensity compared to control in both cell lines. There was a marked and dose-dependent increase of the signal strength of both fractions when OMP was used. 5-FU alone did not relevantly influence the LC3-level. The corresponding β-Actin level is shown below the LC3-WB to confirm the correctness of this semiquantitative evaluation.