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. 2011 May 24;6(5):e20276. doi: 10.1371/journal.pone.0020276

Figure 2. 5-FU-induced down-regulation of CALB2 is caspase-independent.

Figure 2

p53+/+ HCT116 cells were treated with an ∼IC60(72 h) dose of 5-FU alone or combined with a 10 µM dose of pan-caspase inhibitor Z-VAD (OMe)-FMK (Z-VAD) for 72 h (A) Caspase 3/7 activity was quantified using a luciferase reporter based assay. Values are RFU read-outs relative to cell viability, as determined by MTT. (B) Real-time RT-PCR quantification of CALB2 mRNA (fold-change relative to the untreated, time-matched control). (C) Western blot analysis of CALB2 expression and corresponding densitometry data of CALB2 expression relative to the GAPDH loading control.