Table 1. Pathways associated with 5-FU resistance.

	Number of genes passing filter of 1.5 fold-change and p<0.05
	Inducible parental	Inducible resistant	Constitutively de-regulated
Apoptosis	7	7	5
Calcium signalling	8	7	9
Cell cycle	21	13	11
Focal adhesion	16	13	10
Insulin signalling	20	12	8
MAPK signalling	19	14	12
Purine metabolism	19	12	17
Pyrimidine metabolism	9	12	10
Regulation of actin cytoskeleton	15	16	8
Tight junction	6	14	7
Wnt signalling	12	8	8

KEGG pathway analysis was used to identify pathways from 5-FU in vitro genelists and the pathways common to all genelists were selected. The 5-FU in vitro genelists were: (1) genes induced by 5-FU in the sensitive HCT116 parentals (inducible parental), (2) genes induced by 5-FU in the 5-FU-resistant HCT116 sub-line (inducible resistant) and (3) constitutively de-regulated genes in the 5-FU-resistant sub-line compared to the sensitive parentals (constitutively deregulated). Pathways contained genes passing a 1.5 fold change and t-test (p<0.05).