Skip to main content
. 2011 May 24;6(5):e20352. doi: 10.1371/journal.pone.0020352

Figure 1. Expression of Lc-targeting TFBs containing various truncations of TrCP cDNA.

Figure 1

(A) Schematic of various expressed TFB fusion proteins containing different regions of TrCP cDNA including the F-box domain (scale approximate). All fusion proteins contain a streptavidin binding peptide (SBP) and a yellow fluorescent protein (YFP) domain at the amino end. Each TFB contains the BoNT/A Lc binding VHH, ALcB8, or the BoNT/B Lc binding VHH, BLcB10. Some proteins contain one or more WD40 repeat (w). (B) Western blot of recombinant TFBs expressed in M17 cells. M17 cells were transfected with expression plasmids for the different ALcB8 TFB protein diagrammed in A. The proteins were affinity purified with GST-ALc, resolved by SDS-PAGE and detected by Western blot with anti-GFP antibody (Santa Cruz). Data shown is representative of three separate experiments. The 117 kDa B8-TrCP and B8-D1 proteins were apparent on longer exposures.