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. 2011 May 24;6(5):e20352. doi: 10.1371/journal.pone.0020352

Figure 2. BoNT Lc targeted TFBs reduce the steady-state level of co-expressed Lc in transfected neuroblastoma cells.

Figure 2

N2A cells were co-transfected with expression vectors for CFP-ALc or CFP-BLc and for TFB D5-B8 or D5-B10 as indicated. (A) Western blots. 24 hrs post-transfection, cell extracts were prepared and resolved by SDS-PAGE. CFP-Lc and SNAP25 expression levels were detected by Western blotting using anti-GFP or anti-SNAP25 antibody. (B) Capture ELISA. Cell extracts prepared from transfected cells in A were quantified by capture ELISA. Background absorbance was subtracted from the absorbance at OD450 nM. Data are presented as averages ± standard deviation calculated from three independent samples and compared by unpaired t test. The differences between each pairing are highly significant (p<0.001).