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. 2011 May 24;6(5):e20389. doi: 10.1371/journal.pone.0020389

Figure 2. Effect of S1P on mRNA (A) and protein (B,C) expression levels of SM markers in human mesoangioblasts.

Figure 2

Role of S1PR (D). Cells were incubated in DMEM with S1P and analyzed by A) Real-Time PCR; B) Western blot analysis; (lower panel represents densitometric quantification) and C) Confocal microscopy analysis (40× magnification) using TRITC-phalloidin and anti-fluorescein conjugated specific antibodies. D) Cells were pre-incubated with VPC23017 and JTE071 in DMEM before being challenged with S1P and analyzed by Western blot analysis for CNN1 (lower panel represents densitometric quantification).