Skip to main content
. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Mol Cell. 2011 May 6;42(3):356–366. doi: 10.1016/j.molcel.2011.04.010

Figure 4.

Figure 4

Mutation in MEMB12 leads to enhanced resistance to both Pst (EV) and Pst (avrRpt2) and promotes PR1 secretion. (A) Growth of Pst (EV) and Pst (avrRpt2) was measured in four-week-old memb12-1 and corresponding Ler WT as described in figure 1C. Error bars represent standard deviation of six leaf discs (*: p<0.05; **: p<0.01). Similar results were obtained in three biological replicates. (B) memb12-1 promotes PR1 secretion. The level of PR1 in total (T; whole leaf) and secreted proteins (E; intercellular wash fluid) from mock- and Pst (avrRpt2)-treated Ler WT and memb12-1 plants was detected by Western blot analysis. Equal volume of intercellular wash fluid was loaded for detecting secreted PR1. Tubulin was used as an equal loading control and RA measurement for detecting total PR1 protein. Similar results were observed from 4 biological repeats. (C) Secretion of PR1 was reduced and delayed in ago2-1 mutant plants. The total (T) and secreted (E) PR1 were compared between Col-0 WT and ago2 plants at 6, 9 and 12 hpi of Pst (avrRpt2). Similar results were obtained from 3 biological repeats (Fig. S3). See Fig S3 for the characterization of memb12-1 mutant.