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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Mol Cell. 2011 May 6;42(3):356–366. doi: 10.1016/j.molcel.2011.04.010

Figure 5.

Figure 5

MEMB12 is likely to regulate secretion of PR proteins in a rather specific manner. (A) Mutation of another intracellular SNARE gene SYP61 does not interfere with PR1 secretion. Secreted proteins were isolated from syp61 and the corresponding C24 WT and detected as in Fig. 4B. Experiments were performed two times with similar results. (B) MEMB12 has weak effect on secretion of a PM-localized SNARE protein SYP121. PM proteins were fractionated from four-week-old mock- and Pst (avrRpt2)-treated tissue collected at 12 hpi. SYP121 was detected by SYP121 antibody (Collins et al., 2003). PIP-28RD (AT2G37180) was used as a loading control. Similar results were obtained from three biological repeats. (C) Genes involved in protein folding and secretory pathways were coordinately up-regulated in memb12-1. The transcript levels of some reported secretory-related genes (Wang et al., 2005) were detected by real-time RT-PCR. Expression levels in Ler WT plants were assigned as 1.0. Error bars indicate standard deviation from three technical repeats. Similar results were obtained in two biological replicates. See Table S3 for detailed primer and probe sequences.