Table 1.

[1′-³H] BIEs and k_cat/K_m KIEs for PfOPRT and HsOPRT.

		OMP [1′-3H] vs [5′-14C]
		binary complex OPRT•OMP	ternary complex OPRT•OMP•SO42−
BIEsa	PfOPRT	1.104 ± 0.002	1.091 ± 0.003
	HsOPRT	1.108 ± 0.003	1.096 ± 0.004
Kd (μM)	PfOPRT	3.1 ± 1.6	5.6 ± 1.8
	HsOPRT	2.5 ± 1.2	4.1 ± 2.5
		expt	calcd
KIEsb	PfOPRT	1.261 ± 0.014	1.335
	HsOPRT	1.199 ± 0.015	1.330
Kmb (μM)	PfOPRT	3.7 ± 1.4
	HsOPRT	1.6 ± 0.6

		Orotidine [1′-3H] vs [5′-14C]
		binary complex OPRT•orotidine	ternary complex OPRT•orotidine•SO42−
BIEsa	PfOPRT	0.997 ± 0.003	0.991 ± 0.006
	HsOPRT	0.999 ± 0.003	0.991 ± 0.005
Kd (μM)	PfOPRT	130 ± 34	160 ± 30
	HsOPRT	140 ± 27	150 ± 22
		expt	calcd
KIEsb	PfOPRT	1.189 ± 0.006	1.405
	HsOPRT	1.209 ± 0.004	1.411
Kmb (μM)	PfOPRT	96 ± 40
	HsOPRT	91 ± 37

^aBIE ± standard error. Each BIE was measured with at least 4 replicates.

^bFrom references 8 and 9.