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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: Mutat Res. 2011 Jan 7;711(1-2):49–60. doi: 10.1016/j.mrfmmm.2010.12.015

Figure 3.

Figure 3

Illustration of the image analysis and focus identification algorithm. Panel A shows a fragment of an image of mouse tongue section with two nucleus object outlined in light blue colour. Panel D shows the segmentation of the image using the conventional intensity threshold. Application of the Top Hat transformation and subsequent segmentation using H-Dome transformation in combination with the intensity threshold are demonstrated in panels B and E respectively. The structuring element is shown in panel B (top left corner) in pink colour. Panel C illustrates identification of foci, and subsequent application of the object splitting procedure is shown in panel F.