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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: Mutat Res. 2011 Jan 7;711(1-2):49–60. doi: 10.1016/j.mrfmmm.2010.12.015

Figure 4.

Figure 4

Optimisation of the focus analysis parameters. Each curve in panel A represents results of a series of counting runs using various values of the size of the structuring element (Top Hat transformation) for a fixed value of the dome height (H-Dome transformation) for images from blood lymphocytes samples. Open triangles, squares and inverse triangles represent results for the dome height of 40, 50 and 60% for 1.5 Gy sample. Closed triangles show the result for the dome height 40% following additional application of the object splitting procedure. Open circles represent the results for 0.6 Gy sample and the dome height 40%. Panel B shows the average number of potential foci per nucleus counted at various threshold intensity levels for the control image (non-irradiated sample, circles), test image (5 Gy irradiated sample, triangles) and the difference between the test and control focus count (squares) obtained from mouse tongue sections. The threshold is represented as a percentage of the possible maximum image intensity. An optimal value of the threshold is 3 corresponding to the maximum of the difference between the test and control image.