Figure 4. Pairwise comparison of transcriptomes from cells supplemented with different trace element recipes.

A. Logarithmic mean-difference scatterplots of normalized expression fold-change (y axes) vs. mean expression (x axes). The plots were generated from pairwise transcriptome comparisons of wild-type strain 2137 supplemented with Hutner trace element recipe (H₂₁₃₇) (Castruita et al. unpublished), wild-type strain D66 supplemented with Hutner trace element recipe (H_D66) (González-Ballester et al., 2010), and wild type strain 2137 supplemented with an early iteration of the revised trace element mix (mK₂₁₃₇). Cyan lines in scatter plots represent the average fold change. Red and green lines represent the 95^th and 5^th percentile of fold changes, respectively. Gray lines represent a 2-fold difference in fold change. B. Histogram of expression fold-changes for each pairwise comparison grouped into clusters of log₁₀ 0.2.