Table 3.
Relative transcription levels of the acrB, ramA, and soxS genes in the in vitro-selected mutants, their parental strains, and their wild-type ramR or soxR transformants (for ramR and soxR mutants, respectively) in comparison with strain ATCC 138821
| Straina | Gene transcription level (fold change)b |
||
|---|---|---|---|
| acrB | ramA | soxS | |
| ATCC 138821 | 1 | 1 | 1 |
| KPBj1 Rev | 1.15 (±0.15) | 1.05 (±0.44) | ND |
| ramR mutants | |||
| KPBj1 M2 Cip | 7.05 (±1.58) | 31.62 (±16.67) | ND |
| KPBj1 M2 Cip T0 | 5.49 (±3.87) | 7.68 (±1.02) | ND |
| KPBj1 M2 Cip TramR | 1.93 (±0.03) | 0.19 (±0.07) | ND |
| KPBj1 M1 Cip | 3.38 (±0.92) | 43.91 (±30.32) | ND |
| KPBj1 M2 Lev | 4.34 (±2.20) | 38.10 (±9.24) | ND |
| KPBj1 M2 Lev TramR | 1.27 (±0.25) | 0.12 (±0.01) | ND |
| KPBj1 M3 Fox | 5.81 (±2.41) | 87.53 (±42.78) | ND |
| KPBj1 M3 Fox TramR | 1.37 (±0.08) | 0.14 (±0.04) | ND |
| Mutant with unknown mutation | |||
| KPBj1 M1 Lev | 4.01 (±0.40) | ND | ND |
| KPBj3 Rev | 2.20 (±0.57) | 2.31 (±2.92) | ND |
| ramR mutants | |||
| KPBj3 M2 Fox | 9.18 (±0.85) | 37.50 (±18.97) | ND |
| KPBj3 M2 Fox TramR | 1.52 (±0.36) | 0.09 (±0.03) | ND |
| KPBj3 M3 Fox | 6.48 (±1.32) | 13.64 (±8.37) | ND |
| KPBj3 M3 Fox TramR | 1.40 (±0.27) | 0.06 (±0.001) | ND |
| Mutants with unknown mutations | |||
| KPBj3 M1 Cip | 4.04 (±0.40) | ND | ND |
| KPBj3 M2 Cip | 4.94 (±0.07) | ND | ND |
| KPBj3 M1 Lev | 4.54 (±0.26) | ND | ND |
| KPBj3 M2 Lev | 5.50 (±0.06) | ND | ND |
| KPBj5 Rev | 0.97 (±0.05) | 2.59 (±0.34) | 2.78 (±3.17) |
| ramR mutants | |||
| KPBj5 M2 Lev | 5.09 (±2.18) | 84.21 (±72.24) | ND |
| KPBj5 M2 Lev TramR | 1.45 (±0.34) | 0.14 (±0.12) | ND |
| KPBj5 M3 Fox | 3.33 (±0.82) | 80.29 (±60.10) | ND |
| KPBj5 M3 Fox TramR | 1.39 (±0.01) | 0.10 (±0.06) | ND |
| soxR mutant | |||
| KPBj5 M1 Cip | 1.37 (±0.07) | 3.38 (±0.97) | 7.21 (±4.73) |
| KPBj5 M1 Cip TsoxR | 1.66 (±0.11) | 3.99 (±2.70) | 2.34 (±0.33) |
| Mutants with unknown mutations | |||
| KPBj5 M2 Cip | 2.72 (±0.11) | ND | ND |
| KPBj5 M1 Lev | 3.28 (±0.83) | ND | ND |
| KPBj5 M1 Fox | 9.70 (±3.92) | ND | ND |
a
T0, strain complemented with plasmid pSC-A-amp/kan-E1; TramR, strain complemented with plasmid pSC-A-amp/kan-ramR bearing the wild-type ramR gene; TsoxR, strain complemented with plasmid pSC-A-amp/kan-soxR bearing the wild-type soxR gene.
b
ND, not determined. For gene expression levels, the results of two different experiments are indicated as mean (±standard deviation).