Fig. 4.

Impacts of amphomycin and MX-2401 on bactoprenol-phosphate-consuming reactions catalyzed by MraY (A) and TagO (B). The incorporation of [¹⁴C]UDP-MurNAc-pentapeptide into lipid I (A) and [¹⁴C]UDP-GlcNAc into lipid III (B) was analyzed in the presence of increasing peptide concentrations. Peptides were added in molar ratios of 0.25 to 2 with respect to the substrate C₅₅-P. Daptomycin was added in 2-fold molar excess with respect to C₅₅-P. Quantification was carried out using phosphorimaging. The error bars indicate standard deviations.