Fig. 3.

Specific binding of MtrR upstream of glnA. The area of MtrR-specific binding was identified using the three sections of the full-length DNA sequence described in Fig. 1. The full-length probe was PCR amplified from DNA of strain FA19 using primers 5′pglnA and 3′pglnA (Table 2), while binding site I was amplified with primers glnAsec1F and glnAsec1R. The nonspecific probe was prepared using primers rmpF and rmpR. MtrR was found to specifically bind only (data not presented) to section I since specific competitor DNA, but not nonspecific competitor (up to 100 times), was able to abrogate the shifting of section I glnA DNA in EMSA; this result is shown in the figure.