Fig. 5.

MtrR influences FarR::DNA complexes. Shown are the results from an EMSA experiment that evaluated the binding of MtrR and FarR to the ³²P-labeled full-length glnA probe (Fig. 1) that was prepared as described in the legend to Fig. 3. The lane assignments are as follows, from left to right: probe alone; probe plus 0.1 μg of MtrR-MBP; probe plus 0.1 μg of MtrR-MBP and increasing amounts of FarR-His (0.05, 0.1, 0.25, 0.5, 1.0, and 2.0 μg); probe alone; and probe plus 0.5 μg of FarR-His with increasing amounts of MtrR-MBP (0.05, 0.1, 0.25, 0.5, 1.0, and 2.0 μg). The positions of MtrR and FarR shifted bands in the absence of competing protein are shown.