Table 2.
Oligonucleotides used in this study
| Oligonucleotide useda | Sequence (5′ → 3′) |
|---|---|
| 5′pglnA | GGGGATCCCATAAAGGCGGGGCGGTC |
| 3′pglnA | GGGGATCCCGGGACATCTTCAGCTCCTGAA |
| glnA1 | GCAACCGCCTGTTTCAAAAAATG |
| glnA2 | GGACATCTTCAGCTCCTGAAAAAG |
| glnAsec1F | TAACGTTTGCCCCGCAACC |
| glnAsec1R | CCCCCGCTACGCCGTTTTC |
| glnAsec2F | GGGCGTGCATAGTCATATTC |
| glnAsec2R | GCGTCAAATTCCAAGCCGG |
| glnAsec3F | AAACCGGTTTCAGACGGCAT |
| glnAsec3R | GGACATCTTCAGCTCCTGAA |
| glnAtrunckf | CATGGATCCGATGAATCTGCGGCGATTTG |
| rmpF | CATGTTTCTACAGCGGCCTG |
| rmpR | CGGCAAGATATTACCTAGCCT |
| 16SRTR | CATCGGTATTCCTCCACATCTC |
| 16SRTF | GTAGGGTGCGAGCGTTAATC |
| glnARTR | GGCTTTGGCGTGTTTGATG |
| glnARTF | TCCGATACCGCGCTCTACTAC |
a
Primers with ending letters RTR or RTF were used in qRT-PCR for the 16S rRNA and glnA transcripts.