Table 2.
Primers used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| Vrasr-up-f | AAAGGTACCTTGGATACATATGCGCTAAAGTGCGC |
| Vrasr-up-r | AAATCTAGAGAATAGGCTCCGGAACTAAATGATG |
| Vrasr-down-f | AAATCTAGACAATAGTTCGTATCGAATTAAG |
| Vrasr-down-r | AAACTGCAGAGGTTAAGGAGTGCACATGTCG |
| CmR-f | AAATCTAGACGCCGGCAATAGTTACCC |
| CmR-r | AAATCTAGAGGATATGGATCTGGAGCTG |
| SAV1889-f | CAGCTGTTAAAATGGCATAGATGGG |
| SAV1882-f | AATCCTGAATTAGAACGTTC |
a
Underlined sequences indicate restriction enzyme recognition sequences for KpnI (GGTACC), XbaI (TCTAGA), or PstI (CTGCAG).