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SDS-PAGE gel of BIO-AMP-labeled GST-PBP2a (left) and untagged PBP2a (right), as a function of the BIO-AMP concentration relative to the Kmapp for BIO-AMP (as determined against GST-PBP2a in the microtiter plate-based assay). Some untagged PBP2a was present in the GST-PBP2a preparation due to the presence of endogenous proteases during GST-PBP2a purification.
