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. 2011 Jun;55(6):2552–2558. doi: 10.1128/AAC.00109-11

Fig. 3.

Fig. 3.

HPLC analysis of enzyme-bound feedback inhibitor. (A) HPLC standards; (B) recombinant human TK2; (C) recombinant human TK2 (20 μM) incubated with 200 μM dThd or AZT; (D) recombinant human TK2 incubated with dCyd. After dialysis, the protein was precipitated with 10% PCA and removed by centrifugation, and the supernatant was analyzed by HPLC after neutralization. Abs, absorbance.