Table 1.
aNT, nontypeable. A weak band was observed in some cases.
bB(L) signifies that the size of the DNA fragment amplified by a primer pair identifying the mecA gene lineage as IS1272 was longer than that of standard strains carrying the class B mec gene complex. A long-range PCR using primers mA7 and IS7 for the class B mec gene complex was performed, which produced a 4,159-bp amplicon, compared to a 2,827-bp amplicon produced by the standard strain, CA05. A 4,159-bp amplicon (GenBank accession no. HQ157182) containing a free copy of IS256 was inserted 45 bp downstream of the mecRI start codon on the opposite strand, which generated an 8-bp target site duplication (CATTGCTC) upon transposition.