Table 2.

Primers used in this study

HJL designation	Primer	Sequence (5′–3′)a	Application
HJL1205	CtFCY1-f	ATCATTAGTTCAGATGGTAAAGTCTTG	PCR and sequencing
HJL1206	CtFCY1-r	CCTTTTTAGTAACATGTCTATTCTCCA	PCR and sequencing
HJL1211	CtFUR1-f	TCATCAAAACCATGTCTGCTG	PCR and sequencing
HJL1212	CtFUR1-r	AAGTGTATGTAGTGATAATTGCTATGC	PCR and sequencing
HJL1413	CtURA3-f	ATTGGATAGTCCCTCTAAACTCACTACTA	PCR and sequencing
HJL1414	CtURA3	AGCATTAGTTATATCACTCCACGATGAA	Sequencing
HJL1415	CtURA3	TGCCGATATTGGAAATACAGTTA	Sequencing
HJL1416	CtURA3-r	AATCAACTATTCAAGTTGACCG	PCR and sequencing
HJL814	CaSAT1-f	CTCAACATGGAACGATCTAGC	PCR
HJL1207	CtFCY2-f	TGCCCATAAATTAAATGCAGAA	Sequencing
HJL1208	CtFCY2-r	GGAAGCAACAAACCCAAAAA	Sequencing
HJL1209	CtFCY2-f	TGCTGCCGATTATGTTGTTT	Sequencing
HJL1210	CtFCY2-r	GTGAAAACGAGCCAATCCAT	Sequencing
HJL1420	CtFCY2-f (KpnI)	ggtaccTCAACTCAACCCCAAAGT	Fusion PCR and sequencing
HJL1421	CtFCY2-r (XhoI)	ctcgagCCCAAGGAGAAAGTAGCA	PCR
HJL1422	CtFCY2-f	CGGATTCAATGTAGCCAG	PCR
HJL1423	CtFCY2-r	GTCATTCCATGTCGTGGT	PCR
HJL1424	CtFCY2-r (XhoI)	ctcgagGTCATTCCATGTCGTGGT	Fusion PCR and sequencing
HJL1477	CtFCY2-r out of B (3′)	CTGTTGCTCCAGGTGAATCA	PCR
HJL1753	CtFCY2f	TCGTTGCTTGTGTTGGTTGG	Sequencing
HJL2100	CtFCY2-145Gf	CATAAATTAAATGCAGAAACTAAAGGTATTG	Fusion PCR
HJL2101	CtFCY2-145Gr	CAATACCTTTAGTTTCTGCATTTAATTTATG	Fusion PCR
HJL2102	CtFCY2-145Tf	CATAAATTAAATGCATAAACTAAAGGTATTG	Fusion PCR
HJL2103	CtFCY2-145Tr	CAATACCTTTAGTTTATGCATTTAATTTATG	Fusion PCR
HJL2104	CtFCY2-f	CTTCTCCTTAACTACCTTTTCCTCC	Sequencing

^aRestriction enzyme sites are indicated by lowercase letters; mutation sites are indicated by underlining.