Table 2.
Oligonucleotides used in this study
| Primer | Gene | Oligonucleotide sequence (5′–3′) | Positiona | Purpose | Source or reference |
|---|---|---|---|---|---|
| ampC1 | blaCMY | ATG ATG AAA AAA TCG TTA TGC | 1–21* | PCR, sequencing | 11 |
| ampC2 | blaCMY | TTG CAG CTT TTC AAG AAT GCG C | 1122–1143* | PCR, sequencing | 11 |
| ampC5 | blaCMY | CAG CGT TTG CTG CGT G | 222–237* | PCR, sequencing | This study |
| E4/F | blaCMY | TGG GTT CAG GCC AAC ATG GAT GC | 757–779* | PCR mapping, sequencing | This study |
| E7/R | blaCMY | TGC CAG CAT CAC GAT GCC AAG G | 1059–1080* | PCR mapping, sequencing | This study |
| ecpF2 | 3′ end of ISEcp1 | GTT GCT CTG TGG ATA ACT TG | 2084–2102† | PCR, sequencing | 39 |
| ecpR1 | ISEcp1 | CCT AAA TTC CAC GTG TGT | 2247–2264† | PCR | This study |
| ISEcp1/F | tnpA of ISEcp1 | CAT GCT CTG CGG TCA CTT C | 959–977† | PCR mapping | This study |
| E8/F | Downstream of blaCMY | CCA GGA TAT TGG GCC TC | 3534–3550† | PCR mapping | This study |
| blc/R | blc | GAC AAC CAG GAA TGC AGC | 3647–3664† | PCR mapping | This study |
| sugE/R | sugE | GCC TGA TAT GTC CTG GAT CGT | 4448–4468† | PCR mapping | This study |
| ORF6/R | orf6 | CTT CAT CCC TAT CAT CGC CA | 5634–5653† | PCR mapping | This study |
| ORF6e/R | orf6 | AAT CAG CAA TAA CAT CAC CAT G | 5770–5791† | PCR mapping | This study |
| mobB/R | mobB | TAG AGC AGC AGA AGC CAG CT | 6205–6224† | PCR mapping | This study |
| RNAIIp/R | RNAII | TCA TTC CAC GCC TGA CAC TC | 6741–6760† | PCR mapping | This study |
| pepQ/F | pepQ | CAC CTG TTG ATT ATT GGC AT | 3872704–3872723‡ | PCR mapping, sequencing | This study |
| pepQ/R | pepQ | TCA GGC TAA ATG TAA GTC TC | 3873769–3873787‡ | PCR mapping, sequencing | This study |
| blc/F | blc | CTG TGG ATC CTT TCA CGG A | 4025–4043† | Inverse PCR | This study |
| C12-tnpA/r | tnpA of ISEcp1 | TAT TCT GAA GAG TCC AAG GAA | 1921–1901† | Inverse PCR | 6 |
| ppiD/F | Intergenic region PMI0120/ppiD | GCG ATT ACT GAA TGC CAT C | 150710–150692‡ | PCR mapping, sequencing | This study |
| ppiD/R | ppiD | CAA CGG CAG AAC AGC TTG | 150366–150383‡ | PCR mapping, sequencing | This study |
a
*, Position numbering is according to the first nucleotide of the coding sequence of the blaCMY gene; †, position numbering is according to the sequence of plasmid pH 205 (GenBank acc. no. EU331426) (39); ‡, position numbering is according to the sequence of the P. mirabilis HI4320 genome (GenBank accession no. AM942759) (28).