Figure 1.

Functional Analysis of BRI1 Phosphorylation Sites.

The bri1-5 receptor kinase mutant is a weak allele with semidwarf phenotype, altered leaf structure, and shortened petioles. Expression of wild-type BRI1-Flag in bri1-5 rescues the mutant phenotype, while expression of a mutant construct in which the critical Thr-1049 kinase domain activation loop phosphorylation site is substituted with Ala leads to a dominant-negative effect with an extreme dwarf phenotype similar to bri1 null mutants. (Adapted from Figure 8 of Wang et al. [2005b] with permission.)