Figure 2.

Localization of CPR Swapping Mutants in Protoplasts.

(A) Sequences of CPR swapping mutants. CPRs were swapped between ER and chloroplast/mitochondria C-SA proteins and the resulting constructs were fused to GFP. Localizations listed in the table are from results shown in (B). Underlined and bold sequences are TMD and CPR, respectively.

(B) Targeting of CPR swapping mutants. The CPR swapping mutants in (A) were introduced into protoplasts together with OEP7:RFP or BiP:RFP, and their localization was examined under a fluorescent microscope. Mitotracker was used to stain mitochondria. To simplify the labels, GFP was omitted from the construct names throughout. Bars = 20 μm.