Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 5;23(4):1404–1415. doi: 10.1105/tpc.110.080721

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 American Society of Plant Biologists

PMC Copyright notice

Figure 7. — ABI3 and PIL5 Interact at the Protein Level.

(A) In vitro binding assay showing the protein–protein interaction between ABI3 and PIL5. MBP-ABI3, MBP-fused ABI3 protein; PIL5H, His-tagged PIL5 protein; ABI5H, His-tagged ABI5 protein; IN, 5% of input proteins.

(B) ChIP assay showing the similar enrichments of SOM promoter fragments by ABI3-flag both in the ABI3-OX and ABI3-OX pil5 lines. F1 and F5 are as indicated in Figure 4A. The values shown are immunoprecipitated DNA/input DNA relative to F1. Error bars are sd (n = 3).

(C) ChIP assay showing that PIL5-myc enriches SOM promoter fragments to a similar extent both in the PIL5-OX3 and PIL5-OX3 abi3-6 lines. F1 and F5 are as indicated in Figure 4A. The values shown are immunoprecipitated DNA/input DNA relative to F1 (sd, n = 3).