Figure 3.

WRKY33 Induction after B. cinerea Infection Is Dependent on the MPK3/MPK6 Cascade.

(A) Induction of WRKY33 gene expression was compromised in the rescued mpk3 mpk6 double mutant. Wild-type (Col-0), mpk3, mpk6, and mpk3 mpk6 seedlings were inoculated with B. cinerea. Samples were collected at indicated times. Induction of the WRKY33 transcript was quantified by real-time qPCR. Error bars indicate se (n = 3).

(B) and (C) Activation of camalexin biosynthetic genes, including CYP71A13 (B) and PAD3 (C), was compromised in the mpk3 mpk6 mutant. Error bars indicate se (n = 3). Asterisks above the columns indicate the data sets that are statistically different from those without asterisk at a specific time point (P value < 0.05).