Figure 9.

WRKY33 Binds to Its Own Promoter and the Promoter of PAD3 in Vivo.

ChIP-qPCR analysis was performed using DD/4myc-WRKY33^WT plants generated from the cross of wrky33/4myc-WRKY33^WT with DD lines. Input chromatin was isolated from 2-week-old seedlings 12 h after DEX treatment. Epitope-tagged WRKY33-chromatin complex was immunoprecipitated with an anti-myc antibody. A control reaction was processed side-by-side using mouse IgG. ChIP- and input-DNA samples were quantified by real-time qPCR using primers specific to the promoters of WRKY33 (A) and PAD3 (B) genes. The ChIP results are presented as percentage of input DNA. Error bars indicate se (n = 3).