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. 2010 Sep 20;190(6):1023–1037. doi: 10.1083/jcb.201003122

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© 2010 Winslow et al.

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Figure 2. — Overexpression of α-synuclein inhibits secretion and causes Golgi fragmentation in mammalian cells. (A) Bar graph indicating the effect of α-synuclein–CFP overexpression on constitutive secretion in a HeLa-derived reporter cell line (C1). Brefeldin A, a known inhibitor of constitutive secretion, was used as a positive control (*, P < 0.05; two-tailed Student’s t test; n = 3). GH, growth hormone. (B) Immunostaining of endogenous GM130 (cis-Golgi marker) in HeLa cells overexpressing α-synuclein–GFP. Images are z-stack projections. Bars, 20 µm. (C) Quantification of α-synuclein effect on Golgi fragmentation (***, P < 0.001; odds ratio; n = 6). (A and C) Error bars represent SEM.