Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 20;190(6):1005–1022. doi: 10.1083/jcb.200912089

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Mari et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 9. — Live cell imaging of an Atg9 reservoir becoming the PAS. Atg9-mChe GFP-Atg8 (MMY120) cells were grown to log phase and transferred to SD-N medium for 20 min before being imaged as described in Materials and methods. Sequential images acquired with a time lapse of 5 s are shown. The arrow highlights the Atg9 reservoirs that ultimately colocalize with Atg8 in the process of becoming the PAS. The complete movie reconstruction is presented in Video 1. An identical result was obtained with cells expressing endogenous Atg9-GFP (FRY172) and carrying the pCumCheV5ATG8415 plasmid, which expresses mChe-Atg8 (Video 2). DIC, differential interference contrast. Bar, 2 µm.