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. 2010 Sep 20;190(6):965–973. doi: 10.1083/jcb.201002075

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© 2010 Krick et al.

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Figure 1. — Macroautophagy and PMN require Cdc48 and Shp1. (a) GFP levels from GFP-Atg8 degradation reflect the autophagic rate. S. cerevisiae cells grown stationary at 23°C were starved at 23 or 38°C and analyzed in immunoblots with antibodies to GFP (top), proaminopeptidase I (middle), and Pgk1 as a loading control (bottom). (b) Quantification of GFP levels, mean and SD, from at least three experiments. (c) Immunoblot measurement of macroautophagy in ubx mutants at 30°C. (d) GFP levels from breakdown of the PMN marker GFP-Osh1 reflect the PMN rate. Cells were treated as in panel a. (e) Measurement of the PMN rate, as in panel d, in ubx mutants at 30°C.