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Effects of Fasudil on expression of F-actin and G-actin. Primary cultures of mouse astrocytes were treated with Fasudil (100 µM, 24 h), stained to identify F-actin (rhodamine-phalloidin; red) or G-actin (Alexa Fluor 488-conjugated DNaseI; green). Scale bar = 50 µm. Graphs demonstrate image analysis of area above threshold for F-actin and G-actin. *Significantly different from control, P < 0.05, Student's t-test. Data represent mean ± SEM (n = 16).
