Figure 8.

Effects of Fasudil on astrocytic morphology, F-actin organization and EAAT distribution. Control astrocytes (A, B, E, F) and cells treated with Fasudil (100 µM, 24 h; C, D, G, H) were stained for EAAT1 or EAAT2 (green) as well as the astrocytic protein GFAP (blue) or rhodamine-phalloidin to label F-Actin (red). In untreated astrocytes, EAAT1 tends to be dispersed over the cell body (* in panel B), while treatment with Fasudil results in a more punctate distribution of EAAT1 with some clustering (arrow in D) and alignment along astrocytic process (arrowheads in C). In contrast, EAAT2 is changed from a relatively uniform distribution over cell bodies († in F) to a more linear arrangement that does not coincide with processes or actin fibres (x in panel H). Scale bar = 30 µm.