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. 2011 Jun;163(3):649–661. doi: 10.1111/j.1476-5381.2011.01253.x

Figure 7.

Figure 7

Effects of cigarette smoke extract (CSE) and acrolein on p38 MAPK (mitogen-activated protein kinase) and ERK1/2 phosphorylation. Representative blots of p38 MAPK immunoreactivity in airway smooth muscle cell cultures treated with CSE (optical density, OD = 0.1) or acrolein (60 µM) and harvested at the indicated time points (A and B, respectively). Representative blots of ERK1/2 (ERK42/44) immunoreactivity in cells treated with CSE (OD = 0.1) or acrolein (60 µM) and harvested at the indicated time points (C and D, respectively). Effects of 1 µM of the p38 MAPK inhibitors SB203580 and SB202190 (E) and of 0.3 mM of N-acetylcysteine (NAC) (F) on p38 MAPK phosphorylation induced by CSE or acrolein. Quantitative densitometry values (fold changes over basal of phospho-p38 MAPK/total p38 MAPK) are reported below each lane.