Figure 7.
Group II mGluRs and GABAARs are not involved in the developmental regulation of hypothalamic expression of glial Cx43. A–F, Western blots for Cx43 (A, B, D, E) and statistical data (C, F) in the developing rat hypothalamus (A–C) and hypothalamic cultures (D–F) are presented. In C and F, statistical analysis is done using the paired Student's t test relative to P15 saline or DIV15 control (mean ± SEM); n = 6 in each group. Data are normalized and analyzed as described in Figure 1 and 2 for Cx36. Cx43 expression increases during development both in vivo and in vitro; however, the increase is not affected by group II mGluR and GABAAR agonists or antagonists (i.e., no statistical significance between the saline/control and experimental groups on P15 or DIV15 is detected). Bic/PiTX+A/C, Bicuculline, picrotoxin, AP5, and CNQX.