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Regulation of Cx36 expression in mature neurons. Pharmacological treatments were conducted in rat hypothalamic neuronal cultures on DIV30–DIV36 followed by assessment of Cx36 protein expression on DIV36. Statistical analysis was done using paired Student's t test relative to control (mean ± SEM); LY3792688, n = 7; Bic/PiTX+A/C (bicuculline, picrotoxin, AP5, and CNQX), n = 5. Data are normalized and analyzed as described in Figure 2.
