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. 2011 Mar 17;52(3):1474–1485. doi: 10.1167/iovs.10-6241

Figure 2.

Figure 2.

Increase in intracellular cAMP levels with forskolin and rolipram. Serum-starved cells were treated with either rolipram (50 μΜ; 30 minutes; lane 3) or Fsk (10 μΜ; 10 minutes; lane 2) alone or pretreated with rolipram for 30 minutes, followed by Fsk for 10 minutes (lane 4). Treatment with Fsk alone increased cAMP levels to 80 ± 12 pmol/mg compared with control (1.67 ± 0.012 pmol/mg). Pretreatment with rolipram increased Fsk-induced cAMP levels to 1200 ± 185 pmol/mg (P < 0.05). Treatment with rolipram alone also led to a measurable increase in cAMP levels (10 ± 0.2 pmol/mg) compared with control. The results of three independent experiments are expressed as mean ± SEM.