Figure 8.

Y-27632 opposes (ET-1)–induced increase in stress fibers and focal adhesions. Treatment of serum-starved cells with ET-1 (100 nM) increased the number of stress fibers compared with untreated cells (B vs. A) and an increase in the staining for phosphorylated MLC (F). There was an increase in the staining for vinculin in the presence of ET-1 (J, arrows). Y-27632 (5 μM) opposed both the basal and the (ET-1)–induced increase in stress fibers (C, D vs. B), pMLC (G, H vs. F) and focal adhesions (K, L vs. J). The images are representative of three independent experiments.