Table 2.
Primary Antibodies Used for Western Blot Analyses
| Antibody | Source | Catalog No. | Isotype | Dilution |
|---|---|---|---|---|
| Oxidative stress-related proteins | ||||
| Catalase | Abcam* | ab1877 | Rabbit | 1/1000 |
| Cytoglobin | Abcam | ab57713 | Mouse | 1/1000 |
| Glutathione peroxidase-1 | Abcam | ab50427 | Goat | 1/1000 |
| Glutathione peroxidase-3 | R&D Systems† | AF4199 | Goat | 1/1000 |
| Glutathione peroxidase-7 | Abcam | ab51948 | Goat | 1/1000 |
| Glutathione reductase | Abcam | ab84963 | Rabbit | 1/1000 |
| IPCEF-1 (PIP3E) | Abcam | ab80817 | Rabbit | 1/1000 |
| Peroxiredoxin-2 | Millipore‡ | 07–610 | Rabbit | 1/1000 |
| Peroxiredoxin-3 | Abcam | ab16753 | Mouse | 1/1000 |
| Peroxiredoxin-5 | Abcam | ab16944 | Mouse | 1/1000 |
| Superoxide dismutase-1 | Abcam | ab52950 | Rabbit | 1/500 |
| DNA damage signaling pathway proteins | ||||
| AIF | Santa Cruz§ | sc-5586 | Rabbit | 1/200 |
| ATM | Cell Signaling‖ | 2873 | Rabbit | 1/1000 |
| DNA-PKcs | Santa Cruz | sc-56090 | Mouse | 1/200 |
| GADD45a | Santa Cruz | sc-796 | Mouse | 1/1000 |
| OGG1/2 | Santa Cruz | sc-12074 | Goat | 1/200 |
| PCNA | Santa Cruz | sc-7907 | Rabbit | 1/200 |
| p53 | Cell Signaling | 9282 | Rabbit | 1/1000 |
*
Abcam, Inc., Cambridge, MA.
†
R&D Systems, Inc., Minneapolis, MN.
‡
Millipore Corp., Billerica, MA.
§
Santa Cruz Biotechnology, Inc., Santa Cruz, CA.
‖
Cell Signaling Technology, Inc., Danvers, MA.