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. 2011 Mar 28;52(3):1762–1769. doi: 10.1167/iovs.10-6134

Figure 2.

Figure 2.

Immunoblots showing downregulation of desmoglein and desmoplakin in the Klf4CN cornea. (A) Immunoblot of the wild-type and Klf4CN whole corneal extract probed with anti-Dsp antibody. (B) The blot in (A) was stripped of the primary antibody and reprobed with anti-Dsg antibody. (C) The blot from (B) was stripped of the primary antibody and reprobed with anti-actin antibody to ensure equal loading. (D) Fold change in expression of Dsg, Dsp-I, and Dsp-II estimated by densitometric scan of the immunoblots in (A–C) after normalizing for loading differences based on actin levels.