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. 2011 Mar 14;52(3):1439–1449. doi: 10.1167/iovs.10-6400

Figure 3.

Figure 3.

Morphology of ImM10 cells in 2D and 3D Differentiation cultures. (A) Experimental design for differentiation cultures in 2D and 3D cultures. (B–D) Morphology of cells in 2D differentiation cultures. (E–G) Morphology of cells in 3D differentiation cultures. (B) DIC image of cells in sphere-forming media for 7 days. (C) After transfer to priming media, spheres adhere to the dish and cells begin to migrate out (arrowhead). (D) After 5 days in differentiation conditions, some cells acquire a more neuronal morphology and extend thin, neurite-like processes (arrowheads). (E) One day after encapsulation in the 3D matrix and culturing in priming media conditions, cells at the margins of the spheres extend processes (arrowhead). (F) After 5 days in priming media, many cells have migrated from the neurospheres (arrowheads). (G) After 5 days in differentiation conditions, most, but not all, cells have migrated from the neurospheres (arrowheads). Scale bars, 100 μm (B–G).