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. 2011 Mar 14;52(3):1439–1449. doi: 10.1167/iovs.10-6400

Figure 4.

Figure 4.

Immunostaining of ImM10 cells in 2D differentiation cultures. (A) Immunolabeling reveals small subsets of cells that express the neuronal gene MAP1. (B) Hoechst labeling. (C) Merge of (A) and (B). (D) Subsets of cells immunopositive for CaBP1 (arrowheads), a gene expressed in OFF cone bipolar cells and subsets of amacrine cells. (E) Hoechst labeling. (F) Merge of (D) and (E). (G) Subsets of cells immunopositive for Goα, a gene expressed in rod bipolar cells and ON cone bipolars. (H) Hoechst labeling. (I) Merge of (G) and (H). (J) Subsets of cells immunopositive for VGLUT1. Intense VGluT1 immunoreactivity is localized at the plasma membranes of juxtaposed cells (arrowheads). (K) Hoechst labeling. (L) Merge of (J) and (K). (A–L) Scale bar (L), 50 μm. (M) Subsets of cells express PAX6. (N) Hoechst labeling. (O) Merge of (M) and (N). (M–O) Scale bar (O), 50 μm.