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α4β1 integrin ligands induce cell spreading when ILK is inhibited. Differentiated A7–1 cells were plated on the CBD in the absence (A, C, E) or presence of the ILK inhibitor KP392 (100 μM; B, D, F); with or without soluble extracellular domain of VCAM-1 (236 nM; C, D); or the IIICS domain of fibronectin (472 nM; E, F). Cells were labeled with phalloidin to visualize F-actin. Bar, 20 μm.
