Figure 1.

Evaluation of DNA and protein synthesis in enucleated porcine and bovine eyes. (A) The freshly enucleated porcine eye within 1 hour of euthanatization was procured and subjected to determination of BrdU. Similar BrdU incorporation estimation was performed with porcine and bovine eyes (72 hours after enucleation) and plotted as a percentage of the maximum incorporation found in the corneas of porcine eyes subjected to BrdU incorporation within 1 hour of enucleation. (B) As indicated, the eyes were subjected to determination of ³⁵S-labeled methionine incorporation. The maximum incorporation found in the corneas of fresh porcine eyes (within 1 hour of enucleation) subjected to ³⁵S-methionine incorporation was taken as 100% and used for plotting. Comparative incorporations of label in porcine and bovine eyes are shown as indicated. Results are the mean ± SD of three independent determinations.