Figure 4.

Representative bovine corneal normalized protein yield and profile after exposure to ammonium hydroxide. (A) Representative protein yield (μg/mg) after exposure of cornea to 0.25 M (▵), 6 M (□), and 11 M (●) concentrations of NH₄OH at the indicated time intervals. The recovered protein amounts estimated by Bradford's method were normalized to the initial amount of wet tissue used. Results are the mean ± SD of three independent estimates. Results were found to be significantly different from 0.0 by the one-sample t-test (*P < 0.05). (B–D) SDS-PAGE profile of total extracted protein after exposure to 11 M (B), 6 M (C), and 0.25 M (D) NH₄OH, respectively. An equal amount of protein (10 μg) for each predetermined time exposure, as indicated, was separated on 10% SDS-PAGE and subsequently stained with Coomassie blue. Arrowheads: protein bands that decrease in intensity after NH₄OH exposure. Protein bands that appear after exposure to NH₄OH in high (thin arrows) and low (thick arrows) molecular weight regions, respectively.