Figure 5.
Representative bovine corneal normalized protein yield and profile after exposure to calcium hydroxide. (A) Representative protein yield (μg/mg) after exposure of cornea to 0.25 M (▵), 6 M (□), and 11 M (●) concentrations of Ca(OH)2 at the indicated time intervals. Recovered protein amounts estimated by Bradford's method were normalized to the initial amount of wet tissue used. Results are the mean ± SD of three independent estimates. Results were found to be significantly different from 0.0 by the one-sample t-test (*P < 0.05). (B–D) SDS-PAGE profile of total extracted protein after exposure to (B) 11 M, (C) 6 M, and (D) 0.25 M Ca(OH)2. An equal amount of protein (10 μg) for each predetermined time exposure, as indicated, was separated on a 10% SDS-PAGE and subsequently stained with Coomassie blue. Arrowhead: protein band that decreased in intensity after Ca(OH)2 exposure. Protein bands that appeared after exposure to Ca(OH)2 in high (thin arrow) and low (thick arrow) molecular weight regions, respectively.