Table 1.

Experimental Subject Data

Contrast Agent	Subject Number	NIR Wavelength
		700 ± 5 nm	750 ± 5 nm	780 ± 5 nm	800 ± 5 nm	850 ± 5 nm	900 ± 5 nm
ICG	7787	x	x		x	x
ICG	7788	x	x	x	x	x	x
ICG	7793		x	x	x	x
ICG	7793		x	x	x	x
ICG	7793		x	x	x	x
	Total	2	5	4	5	5	1
	P	0.0624	0.04965	0.002415	0.12835	0.004213	NA
Nigrosin	7785	x	x	x	x	x	x
Nigrosin	7791	x	x	x	x	x	x
	Total	2	2	2	2	2	2
	P	0.3099	0.2512	0.2891	0.4037	0.1451	0.2383

For ICG experiments, cats could be imaged on multiple experimental dates because of the fast clearance rate and the low toxicity of ICG. Conversely, nigrosin experiments were terminal, which meant that pre-nigrosin versus post-nigrosin conditions could be reported only once per cat. The columns at right show the range of NIR wavelengths that were examined (center wavelength + half-width at half-height). An “x” indicates data were collected for that wavelength. Some wavelengths were not acquired in certain animals because of temporal constraints of the ICG infusion period that lasted 3 to 4 hours. All wavelengths were collected in the nigrosin experiments because temporal constraints were not an issue (terminal experiment). P values are reported for each wavelength tested (one tailed t-test, where n = number of experiments for a specific wavelength). Significant values (P < 0.05) are plotted as asterisks in Figure 6B. P value is not reported for 900 nm in the ICG condition because only one experiment was performed at that wavelength.