Processing of core glycan is not required for turnover of D316K or K374E. (A) Cells expressing D316K were metabolically labeled for 30 min and either harvested immediately or chased for 6 h. Cells were either untreated (control) or treated with Lac alone (control + Lac), or treated with dNJ or dMJ in the absence or presence of Lac. The percent protein remaining at 6 h of chase is shown for each condition, n = 2. Values shown represent means ± S.D. (B) Cells expressing K374E were labeled for 30 min and either harvested immediately or chased for 24 h. Cells were treated as described in (A). The percent protein remaining at 24 h of chase is shown for each condition, n = 2, except for “control + Lac”, n = 1. Values shown represent means ± S.D., except for “control + Lac”.